The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. A 0. These lab workhorses are used in drug discovery, research, bioassay validation, and. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. DNA extraction from blood meal ELISA homogenate. Each experiment was performed in duplicate and repeated at. 7. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Photometric Range: 0. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Percent viability was calculated by comparison with the control group. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Following cell culture under normoxic or hypoxic con-ditions, culture medium samples were obtained on completing the incubation period to measure atrial natriuretic peptide. Crowder, Ziqian Zeng, Alissa N. SpectraMax® Microplate Readers and SoftMax® Pro Software. Fully tested with Warranty. Softmax PRO v5. Introduction. SpectraMAX 340 microtiter plate reader. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 312 gain calibration failed. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Intra- and inter-assay coefficients of variation were lower than 8. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. Although all samples initially were analyzed at the same volume, the volume of sample. Pharmacokinetic and pharmacodynamic analysis. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. 2. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Molecular Device M2e. Features showcased by this model include. Water – distilled water or equivalent. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The concentration of TNFα in the experimental samples was calculated from a mouse TNFα standard curve of 15–2000 pg/ml. Briefly, cells were seeded into a. SpectraMax ® Plus 384 optics. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. 1x10 5 transduced, GFP + SK-NEP-1. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. No power cord or accessories included. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Molecular Devices. These robust readers have been placed in labs from Antarctica to the. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. 305 reference level saturation. 340/70 nm. Products : SpectraMax Plus 384 reader,. Percent Transmission Absorbance Competitor 0. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. 1 mL of acetylacetone. Product Keyword. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Fig. 0. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. 5% phosphoric acid) in microplates at room temperature for 10 min. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Novak, Nathan J. Microplate Spectrophotometer Operator’s Manual. Setup recommendations for SpectraMax. 6) and 0. Or scan up to 384 wells at any time to determine the best assay conditions. 06 ng/mL; FSH: 0. Assay setup for a 384-well low-volume plate. Thin layer chromatography (TLC) was. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Unit has several areas of damaged plastic. SpectraMax Multi-Mode Microplate Readers. Driessen*,MaaikeA. Microplate Readers. Integration delay. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Manager: Yinghua Chen, PhD Contact Email: yinghua. 303 unable to cal dark current. , Sunnyvale, CA). Molecular Devices SpectraMax M5e Microplate Reader. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). Summary of Contents for Molecular Devices SPECTRAmax 340PC. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Donor: 620 nm. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. The VersaMax and SpectraMax 190 read 96-well microplates. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). For more sample throughput, the system canThe optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Wound healing assay. Number of flashes. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The. 340 nm. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 3. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). 30. Rate of fibrinolysis. 1 0 100 80 60 40 20 0 4. Lactate Dehydrogenase (LDH) Release. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 0 2. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The Catalina. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 6. Supplier: Molecular Devices. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. One plate carrier adapter is provided with the instrument. The reaction was stopped with 1 M hydrochloric acid after 15 min. 2 software was used to measure and calculate of the bioactivity activity studies. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. VersaMax reads 96-well and strip-well plates. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). , Oxon, UK). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). Bertin Precellys Evolution Touch Homogenizer. 2. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. SpectraMax Microplate Readers. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. In vivo anti-malarial assay in mice. Emission. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. We also offer depot Repair Service from our base of operations in Southern California (San Diego). The SpectraMax® iD3 Multi Mode Microplate . The activity of glutathione peroxidase (GPX) was quantified spectrophotometrically at 340 nm, following the method of Takeda et al. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). 340 nm / 70 nm. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). PGD2 concentrations in samples from treated cell cultures were determined using the linear range of a standard curve and are presented as percentages of the PGD2 levels measured in the solvent controls. The assays contained 0. The plate was covered and incubated for one hour at room temperature. Details. 05 ng/mL; progesterone: 2. 0200-0729. 97), and each plate contained at least six positive and six negative controls. 30. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax Gemini XS Microplate Fluorometer $2,800. Quick View. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The membranes were. 190–850 nm <± 1. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San. Details. 5. Assay setup for a 384-well low-volume plate. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Triton X-100 (see Note 2). AoSMCs were seeded into 6-well culture plates. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. All strains were tested three times and the results averaged. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Vessel Sprout Formation Assay. more on but is otherwise untested and sold AS-IS. 314 low lamp level warning. Call 1-800-745-2710. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Absorbance was measured at 450 nm by using a SpectraMax 340 microplate spectrophotometer (Molecular Device). Immunofluorescence and Aggregation Analysis. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Dissolve 13. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. 314 low lamp level warning. Molecular Devices. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SoftMax Pro. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. The cell survival. 0. Or scan up to 384 wells at any time to determine the best assay conditions. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. ). Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Molecular Devices SpectraMax Gemini XS Microplate Reader. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. ). Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax Multi-Mode Microplate Readers. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Acceptor filter: 665/10 nm. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). , Sunnyvale, CA). , Sunnyvale, CA) at 490 nm. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Binding of capture antibodies to polystyrene beads and biotinylation. Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. The ratio of red to green fluorescence intensity was calculated. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Endpoint, kinetic, spectrum. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Labsystems Luminoskan RT Microplate Reader. To measure the cell biomass (g/L),. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. chen@case. All ELISA plates were analyzed using a SpectraMax 340 PC microplate reader (Molecular Devices). Not. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. The contents of the. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. The first time you replace an OEM lamp you must use the N-34-MLA. 312 gain calibration failed. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. albicans pre. Detection limits. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The inhibition percentage was determined by linear regression through ranges of 20 to 30. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Also for: Spectramax 190, Spectramax 340pc384, Versamax. 6 g sodium acetate trihydrate and 2. 00 USD. The slope of the linear portion of the kinetic curve generated from each well was used to. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. To. 30 µs. It includes both a lamp and a new lamp holder. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. 0 to 4. The reaction mixture was incubated for 5 min. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Our absorbance plate readers feature our PathCheck Sensor technology and. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). 5 mL sample or standard solution was well mixed with 0. The plates were incubated for 4 additional hours at 37°C. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. Software (SoftMax Pro ® software or comparable plate reader software). 0200-6117. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. application or budget. 2. Figure 1. 1 mL of culture media with 10% FBS. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. MSD read buffer and TMB one component HRP substrate are from MSD and SurModics (Eden Prairile, MN), respectively. #N207) LAL Reagent Water (Lonza cat. Stat Fax 4700 Microstrip Reader. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Spectral Range. . . Here, we show how the SpectraMax® i3x and SpectraMax® iD5 Multi-Mode Microplate Readers are used to perform robust, no-wash saturation binding assays using HTRF. ) at 490 nm. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. The values were corrected for nonspecific background by subtracting the OD 650. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. 5. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 7. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). 8. 2. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 8 (w) x 15 (d) Performance Specifications Wavelength Range: 340 – 850 nm Wavelength Selection: Monochromator, tunable 1. SpectraMAX 340. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. \r plates can be used in the SPECTRAmax PLUS 384. mums. 1% PEG 8000 at 37°C. control. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Call 1-800-745-2710. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). 315 can't find zero order. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Spectrophotometer (SpectraMax 340). Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Temperature Range: Ambient + 4 °C up to 45 °C. Immunofluorescence and Aggregation Analysis. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. They are for reference only. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. 0 1. 303 unable to cal dark current. This reader only allows high performance HTRF measurement when assays are run in. The sensitivities of the hormones are Prolactin: 0. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. Amino Acid Analysis. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. The growth. Versteegh, Yoran H. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Includes: SpectraMax 340PC384. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The VersaMax and SpectraMax 190 read 96-well microplates. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. The VersaMax and SpectraMax 190 read 96-well microplates. Warranty. Inter-assay variation was prevented by testing all of the samples on the same day. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection.